Telomeric Repeat Binding Factor 1 Transcription Factor Activity Assay

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TFAB00073
€699

Description

ELISA Kit Technical ManualMSDS

Telomeric Repeat Binding Factor 1 Transcription Factor activity assay

Product Description:The ELISA Genie Telomeric Repeat Binding Factor 1 transcription factor activity assay Kit components necessary for detection and qualitative analysis of Telomeric Repeat Binding Factor 1 active transcription factors in eukaryotic nuclear or cell lysates. ELISA Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:NIMA-interacting protein 2;TTAGGG repeat-binding factor 1;Telomeric protein Pin2/TRF1
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4°C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

Telomeric Repeat Binding Factor 1 Transcription Factor activity assay Protein Information

UniProt Protein Function:TRF1: a telomeric repeat binding factor. Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Involved in the regulation of the mitotic spindle. Expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. Two splice variant isoforms have been described.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; nuclear chromosome, telomeric region; cytoplasm; nucleolus; spindle; nucleus

Molecular Function:protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; microtubule binding; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding; DNA bending activity

Biological Process: response to drug; mitosis; positive regulation of mitosis; negative regulation of telomere maintenance via semi-conservative replication; positive regulation of apoptosis; positive regulation of microtubule polymerization; telomere maintenance via telomerase; positive regulation of mitotic cell cycle; negative regulation of telomerase activity; cell division; negative regulation of DNA replication; telomeric loop formation; mitotic cell cycle spindle assembly checkpoint; age-dependent telomere shortening; G2/M transition of mitotic cell cycle; negative regulation of telomere maintenance via telomerase; telomere maintenance; protein homooligomerization

NCBI Summary:This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
UniProt Code:P54274
NCBI GenInfo Identifier:206729904
NCBI Gene ID:7013
NCBI Accession:P54274.3
UniProt Secondary Accession:P54274,Q15553, Q8NHT6, Q93029, A7XP29,
UniProt Related Accession:P54274
Molecular Weight:439
NCBI Full Name:Telomeric repeat-binding factor 1
NCBI Synonym Full Names:telomeric repeat binding factor (NIMA-interacting) 1
NCBI Official Symbol:TERF1
NCBI Official Synonym Symbols:TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
NCBI Protein Information:telomeric repeat-binding factor 1; NIMA-interacting protein 2; telomeric protein Pin2/TRF1; TTAGGG repeat-binding factor 1
UniProt Protein Name:Telomeric repeat-binding factor 1
UniProt Synonym Protein Names:NIMA-interacting protein 2; TTAGGG repeat-binding factor 1; Telomeric protein Pin2/TRF1
Protein Family:Telomeric repeat-binding factor
UniProt Gene Name:TERF1
UniProt Entry Name:TERF1_HUMAN
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