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SuperSet Accessory Pack

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SKU:
RGDC00001
$284

Description

Accessory Pack for SuperSet ELISA Kits

Complementary materials and reagents needed for use with SuperSet ELISA development kits from ELISA Genie. Reagents are provided to be sufficient for 5 plates.

Material / ReagentQuantityDescription
96 wells microtiter plate 5 12 removable strips
Plastic plate covers 10 Adhesive sealers.
    Cover the plate during assay incubations
Plate storage material 5 Plastic bag and desiccant to store plate once coated
    (up to 12 months at 2-8°C)
Reconstitution Buffer (0.1ml) 1 10X concentrate.
    Add 0.9 ml of distilled water directly in the vial
    See section 9.3 of the ELISA SuperSet Insert
Coating Buffer (5ml) 1 10X concentrate. Sterile
    For one plate, add 1 ml to 9 ml of distilled water See section 8.1 of the ELISA SuperSet Insert
Wash Buffer (25ml) 1 200X concentrate
    For one plate, add 5 ml to 995 ml of distilled water
Blocking Buffer (10ml) 5 Ready to use. Sterile
    See section 8.2 of the ELISA SuperSet Insert
    To be discarded and not washed after the addition
Standard Dilution Buffer (12.5ml) 1 10X concentrate
    For one plate, add 2.5 ml to 22.5 ml of distilled water See section 9.2 of the ELISA SuperSet Insert
    To be used for the dilution of samples if necessary
Secondary Antibody Dilution Buffer (25ml) 1 Ready to use
    See section 9.3 of the ELISA SuperSet Insert
HRP Dilution Buffer (25ml) 2 Ready to use
    See section 9.4 of the ELISA SuperSet Insert
Stop Reagent (10ml) 5 Ready to use 1N Sulfuric Acid Solution

Storage Instructions:

  • Store unopened accessory pack at 2-8°C.
  • All reagents should be warmed to room temperature before use. If crystals have formed in concentrated Buffer (Wash and Standard Dilution): warm it gently until they have completely dissolved.
  • Immediately after use remaining reagents and should be returned to cold storage (2-8°C).
  • Once prepared, store diluted buffers 1X at 2-8°C for up to one week.
  • Expiry of the reagents is stated on box front labels.
  • Do not use components past the expiration date.

Safety & Precautions

  • The Stop Solution provided is an acid solution.
  • Reconstitution, Standard Dilution, Secondary Antibody Dilution and HRP Dilution Buffers contain ProClin which may cause an allergic skin reaction.
  • Wear appropriate clothing, gloves and eye protection.
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