STAT1 (Phospho-Ser727) Transcription Factor Activity Assay

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TFAB00056
€699

Description

ELISA Kit Technical ManualMSDS

STAT1 (Phospho-Ser727) Transcription Factor activity assay

Product Description:The ELISA Genie STAT1 (Phospho-Ser727) transcription factor activity assay Kit components necessary for detection and qualitative analysis of STAT1 (Phospho-Ser727) active transcription factors in eukaryotic nuclear or cell lysates. ELISA Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:Transcription factor ISGF-3 components p91/p84
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse, Rat
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4°C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

STAT1 (Phospho-Ser727) Transcription Factor activity assay Protein Information

UniProt Protein Function:STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: axon; cytoplasm; cytosol; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function:double-stranded DNA binding; enzyme binding; identical protein binding; protein binding; protein homodimerization activity; signal transducer activity; transcription factor activity; tumor necrosis factor receptor binding

Biological Process: apoptosis; blood circulation; defense response to virus; endothelial cell migration; JAK-STAT cascade; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of viral protein levels in host cell; positive regulation of mesenchymal cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to cytokine stimulus; response to peptide hormone stimulus; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway; viral reproduction

Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c

NCBI Summary:The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P42224
NCBI GenInfo Identifier:2507413
NCBI Gene ID:6772
NCBI Accession:P42224.2
UniProt Secondary Accession:P42224,Q53S88, Q53XW4, Q68D00, Q9UDL5, A8K989, B2RCA0 D2KFR8, D3DPI7,
UniProt Related Accession:P42224
Molecular Weight:
NCBI Full Name:Signal transducer and activator of transcription 1-alpha/beta
NCBI Synonym Full Names:signal transducer and activator of transcription 1
NCBI Official Symbol:STAT1
NCBI Official Synonym Symbols:CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
NCBI Protein Information:signal transducer and activator of transcription 1-alpha/beta
UniProt Protein Name:Signal transducer and activator of transcription 1-alpha/beta
UniProt Synonym Protein Names:Transcription factor ISGF-3 components p91/p84
Protein Family:Signal transducer and activator of transcription
UniProt Gene Name:STAT1
UniProt Entry Name:STAT1_HUMAN
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