SARS-CoV-2 Recombinant Proteins

COVID-19 Background: SARS-CoV-2 Structure

SARS-CoV-2 is the causative viral agent of the disease COVID-19. It bears several key proteins within its structure which have been the focus of research the past number of months due to their potential to be therapeutic targets in COVID-19.

Some of the key proteins within the structure of SARS-CoV-2 include:

Spike (S) Protein

The spike protein is the one of the four main proteins found on the surface of the coronavirus virion. These transmembrane glycoprotein spikes consists of two components; S1 and S2. S1 and S2 proteins have been shown to play a major role in receptor binding and membrane fusion. The receptor-binding domain (RBD) of the S protein can be found at the N-Terminal end of the S1 domain. It is responsible for host cell entry of the virus via the ACE2 receptor. Upon binding of the Spike S1 domain to ACE2 a conformational change is initiated in the Spike S2 domain; this facilitates the attachment of the virus to the host cell membrane which results in subsequent nucleocapsid transfer into cell.

ACE2 Protein

Angiotensin II converting enzyme (ACE2), a carboxypeptidase, is an enzyme involved in the renin-angiotensin system (RAS). It has been shown to play an important role in regulating the heart function. ACE2 is a receptor of human coronaviruses, such as SARS and HCoV-NL63. This receptor facilitates the uptake of SARS-CoV-2 into host cells. ACE2 is expressed in multiple locations and cell types, notably including alveolar epithelial cells, surface enterocytes within the small intestine, and vascular endothelial cells of the lung, kidneys and heart.

Nucleocapsid (N) Protein

Coronavirus N protein is the most abundant protein expressed on the surface of the coronavirus. It is required for coronavirus RNA synthesis and packaging, and has RNA chaperone activity that may be involved in template switch. The N has also been suggested to play a role in virus particle release.

Envelope (E) Protein

The envelope protein is one of the four integral proteins found on SARS-CoV-2 viral particles. The protein has a central role in virus morphogenesis and assembly, acting as a viroporin and self-assembling in host membranes to form pentameric protein-lipid pores that allow ion transport. The E protein also plays a role in the induction of apoptosis (by similarity).

ELISA Genie SARS-CoV-2 Recombinant Proteins

With the rapid progression of the global pandemic, COVID-19, over the past number of months, there must come innovative drug development and research strategies to battle and overcome this infectious disease.

At ELISA Genie we have developed 58 novel recombinant proteins to aid COVID-19 Research.

Why choose ELISA Genie SARS-CoV-2 recombinant proteins?

Our proteins are highly pure, ranging between 90-98% in purity. They are also physiologically relevant; ELISA Genie recombinant proteins are expressed in mammalian expression systems, meaning that they are synthesized, processed, and secreted in a manner similar to in humans. For this reason, as well as the fact they have been shown to have low endotoxin, our recombinant proteins do not elicit an immune response in vivo. This makes ELISA Genie recombinant proteins hugely relevant to the area of vaccine development for COVID-19.

Types of SARS-CoV-2 Recombinant proteins available

We have synthesized recombinant versions of the key proteins involved in the process of viral infection by COVID-19. These include the integral proteins expressed on the surface of the coronavirus; spike (S) protein, envelope (E) protein, and nucleocapsid protein (N).We have also created recombinant proteins of other proteins involved in the mechanism of infection with coronavirus. These include proteinases, proteases, and helicases essential for coronaviral replication and host cell invasion, as well as human proteins implicated in coronavirus uptake into host cells, such as CD147/Basigin Protein, APN Protein, and the human receptor for SARS-CoV-2; ACE2,

Our recombinant proteins allow scientists to both recapitulate the endogenous activity of key proteins involved in coronavirus infection, as well as develop potential therapies by targeting such proteins.

SKU Product Name Conjugation Region Species

CARP01258

His Tag

Arg319-Phe541

SARS-CoV-2

CARP01259

His Tag

Val11-Arg682

SARS-CoV-2

CARP01260

His Tag

Val11-Gln1208

SARS-CoV-2

CARP01261

His Tag

Gln14-Arg683

SARS-CoV-2

CARP01262

His Tag

Val16-Arg685

SARS-CoV-2

CARP01263

His Tag

Met1-Val75

SARS-CoV-2

CARP01264

His Tag

Met1-Ala419

SARS-CoV-2

RPES0001

His Tag

Ser1-Gln306

SARS-CoV-2

RPES0002

His Tag

Ala1-Gln601

SARS-CoV-2

RPES0003

His Tag

Met1-Gly180

SARS-CoV-2

RPES0004

His Tag

Ala1-Gly638

SARS-CoV-2

RPES0005

His Tag

Ser1-Gln83

SARS-CoV-2

RPES0006

His Tag

Ala1-Gln198

SARS-CoV-2

RPES0007

His Tag

Ala1-Gln139

SARS-CoV-2

RPES0008

Glu1564-Lys1878

SARS-CoV-2

RPES0009

His Tag

Ala1-Gln527

SARS-CoV-2

RPES0010

mFc Tag

Asn331-Val524

SARS-CoV-2

RPES0011

His Tag

Asn331-Val524

SARS-CoV-2

RPES0012

mFc Tag

Arg319-Pro589

SARS-CoV-2

RPES0013

His Tag

Arg319-Ser591

SARS-CoV-2

RPES0014

Avi-His Tag

Arg319-Ser591

SARS-CoV-2

RPES0015

mFc Tag

SARS-CoV-2

RPES0016

Fc Tag

SARS-CoV-2

RPES0017

SARS-CoV-2

RPES0018

His Tag

SARS-CoV-2

RPES0019

His Tag

SARS-CoV-2

RPES0020

His Tag

SARS-CoV-2

RPES0021

His Tag

Met1-Ala419

SARS-CoV-2

RPES0022

His Tag

SARS-Cov-2

RPES0023

SARS-Cov-2

RPES0024

His Tag

Met1-Val75

SARS-Cov-2

RPES0025

His Tag

SARS-Cov-2

RPES0026

Fc Tag

SARS-Cov-2

RPES0027

His Tag

Gln18-Ser740

SARS-Cov-2

RPES0028

Fc Tag

Gln18-Ser740

SARS-Cov-2

RPES0029

Avi-His Tag

Gln18-Ser740

SARS-Cov-2

RPES0030

mFc Tag

Gln18-Ser740

SARS-Cov-2

RPES0031

His Tag

Ala22-His205

SARS-Cov-2

RPES0032

His Tag

Ala22-Arg325

SARS-Cov-2

RPES0033

His Tag

Arg18-Ile339

SARS-Cov-2

RPES0034

His Tag

Met 1-Phe 339

SARS-Cov-2

RPES0035

His Tag

Thr18-Val333

SARS-Cov-2

RPES0036

His Tag

Thr18-Asn334

SARS-Cov-2

RPES0037

His Tag

Lys69­Lys967

SARS-Cov-2

RPES0038

His Tag

Lys69­Ser966

SARS-Cov-2

RPES0039

His Tag

Met1-Ala419

SARS-Cov-2

RPES0040

His Tag

Val16-Pro1213

SARS-Cov-2

RPES0041

His Tag

Arg319-Phe541

SARS-Cov-2

RPES0042

mFc Tag

Arg319-Phe541

SARS-Cov-2

RPES0043

His Tag

Arg319-Phe541

SARS-Cov-2

RPES0044

Fc Tag

Val16-Arg685

SARS-Cov-2

RPES0045

His Tag

Val16-Arg685

SARS-Cov-2

RPES0046

mFc Tag

Val16-Arg685

SARS-Cov-2

RPES0047

His Tag

Ser686-Pro1213

SARS-Cov-2

RPES0048

His Tag

Met1-Ala422

SARS-Cov-2

RPES0049

His Tag

Arg306-Phe527

SARS-Cov-2

RPES0050

His Tag

Met1-Ser740

SARS-Cov-2

RPES0051

His Tag

Glu1564-Val1880

SARS-Cov-2