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Rat Tumor Necrosis Factor Beta (TNFb) SuperSet ELISA Kit

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SKU:
SSRT1056

Description

Rat Tumor Necrosis Factor Beta (TNFb) SuperSet ELISA Kits

The Rat Tumor Necrosis Factor Beta (TNFb) SuperSet ELISA Kit is a development ELISA kit for the detection of Tumor Necrosis Factor Beta (TNFb) in a range of sample types.

The ELISA Genie Tumor Necrosis Factor Beta (TNFb) SuperSet ELISA kits are a range of specially designed components for the development of your very own sandwich ELISA. Each kit contains an optimised antibody pair as well as recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA can also be used for the detection of analytes in more complex matrices such as serum and plasma. As with all ELISA development kits, it is essential to fully evaluate your sample type with the kit prior to experimentation.

Key Features

  • Optimised antibody pair for highly specific and sensitive analyte detection
  • High quality recombinant protein to generate consistent standard curves
  • Maximise laboratory budget with essential ELISA reagents for medium and high throughput analysis
  • Customise your own ELISA for your specific requirements
  • SuperSet ELISA Reagent Kit SSRT1056 enables full and easy assay optimisation
Kit size:20 plates
Range:7.8-500pg/mL
Specificity:3.3pg/mL
Alias:LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha
Plate preparation:1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
SpecificityThe Abs in the kit have high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Beta (TNFb). No significant cross-reactivity or interference between Tumor Necrosis Factor Beta (TNFb) and analogues was observed.
Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Reactivity:Rat
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Additional Information

Reactivity:
Rat
ELISA Type:
SuperSet
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