Rat Tumor Necrosis Factor Alpha (TNFa) SuperSet ELISA Kits
|Kit size:||20 plates|
|Alias:||DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2|
|Plate preparation:||1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.|
2. Aspirate and wash 1 time.
3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
|Specificity||The Abs in the kit have high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa).No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.|
|Sample type:||serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.|