Product Name: Genieplex Rat Th1/Th2/Th17 7-plex Kit
IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha
This GeniePlex Genieplex Rat Th1/Th2/Th17 7-plex Kit is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 7 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
|Analytes:||IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha|
|1x Ab-conjugated beads (premixed):||S4P4 - anti-rat IL-4; S4P6 - anti-rat IL-6; S4P9 - anti-rat IFN-Gamma; S4P12 - anti-rat IL-2; S5P5 - anti-rat TNF-Alpha; S5P7 - anti-rat IL-17A; S5P10 - anti-rat IL-10|
|2x Biotin-detection Ab (premixed):||Biotin - anti-rat IL-4; Biotin - anti-rat IL-6; Biotin - anti-rat IFN-Gamma; Biotin - anti-rat IL-2; Biotin - anti-rat TNF-Alpha; Biotin - anti-rat IL-17A; Biotin - anti-rat IL-10|
|Buffers:||2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent|
|Lyophilized Standard Mix:||Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. One vial containing lyophilized recombinant rat IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNF-Alpha.|
|Sample types:||Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate|
|Sensitivity (LODs):||Rat IFNg,||LLOQs:||Rat IFNg,||ULOQs:||Rat IFNg, >5000 pg/mL; Rat IL-5, >5000 pg/mL; Rat IL-4, >5000 pg/mL; Rat IL-6, >5000 pg/mL; Rat IL-10, >5000 pg/mL; Rat IL-17A, >5000 pg/mL; Rat TNF-alpha, >5000 pg/mL||Standard dose recovery:||Standard dose recovery: 70-130%||Intra-assay CV:||Intra-assay CV: < 10%||Inter-assay CV:||Inter-assay CV: < 20%||Cross-reactivity of analytes in the Panel:||Cross-reactivity of analytes in Rat Group 1: Negligible||Sample volume:||Sample volume: 15 µL/test|