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Rat interleukin 6 ELISA Kit

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SKU:
RTFI00034

Description

Rat interleukin 6 ELISA Kit - Information

The ELISA Genie Rat IL6 ELISA Kit can assay for Rat IL6 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Rat IL6 ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Rat IL6 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Rat interleukin 6 ELISA Kit - Data

DescriptionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Post-Translational Modification
Uniprot IDP20607
Detection methodSandwich ELISA Double Antibody
ApplicationThis immunoassay kit allows for the in vitro quantitative determination of IL-6 concentrations in serum plasma and other biological fluids.
Size96T
Range62.5-4000pg/ml
Sensitivity< 37.5pg/ml
Storage4'C for 6 months
RecoveryMatrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)91-9795
EDTA plasma(n=5)85-10595
UFH plasma(n=5)89-10395
LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:81:16
serum(n=5)88-104%85-105%92-103%90-105%
EDTA plasma(n=5)82-100%83-99%83-100%83-99%
UFH plasma(n=5)80-100%84-96%82-96%85-98%
CV(%)Intra-Assay: CV<8%Inter-Assay: CV<10%
NoteFor Research Use Only
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Additional Information

Reactivity:
Rat
ELISA Type:
Sandwich
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