The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

Rat IFN-gamma ELISA Kit

(No reviews yet) Write a Review
SKU:
RTFI01244

Description

Rat IFN-gamma ELISA Kit - Information

The ELISA Genie Rat IFN-gamma ELISA Kit can assay for Rat IFN-gamma in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Rat IFN-gamma ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Rat IFN-gamma is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Rat IFN-gamma ELISA Kit - Data

Description

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Post-Translational Modification

Uniprot ID P01581
Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of IFN-gamma concentrations in serum plasma and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 9.4pg/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of IFN-gamma and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-gamma in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-104 97
EDTA plasma(n=5) 87-104 95
UFH plasma(n=5) 93-102 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-gamma and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-102% 86-90% 89-104% 89-99%
EDTA plasma(n=5) 82-96% 82-99% 86-101% 87-95%
UFH plasma(n=5) 83-100% 84-94% 83-99% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

View AllClose

Additional Information

Reactivity:
Rat
ELISA Type:
Sandwich
View AllClose