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Rat IFN Beta (Interferon Beta) ELISA Kit

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SKU:
RTFI00889

Description

Product name

Rat IFN-Beta (Interferon Beta) ELISA Kit

Catalogue No.

RTFI00889

Detection method

Sandwich ELISA, Double Antibody

Application

IFN-β ELISA Kit allows for the in vitro quantitative determination of IFN-β concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of IFN-β and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-β in samples.

MatrixRecovery range(%)Average(%)
serum(n=5) 85-98 93
EDTA plasma(n=5) 88-104 94
heparin plasma(n=5) 92-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5) 85-104% 92-105% 92-101% 85-102%
EDTA plasma(n=5) 86-98% 89-101% 84-100% 89-90%
heparin plasma(n=5) 87-99% 80-100% 88-98% 83-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
Rat
ELISA Type:
Sandwich
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