Rat COVID-19 Multiplex Cytokine Immunoassay

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SKU:
RTAMCOV01
€1,999

Description

Rat COVID-19 Multiplex Cytokine Immunoassay

Analytes 

IL2, IL10, GSCF, IP10, MCP1, MIP1A, and TNFα

Species Reactivity:

Rat

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

COVID-19 Multiplex Cytokine Immunoassay is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IL2, IL7, IL10, GSCF, IP10, MCP1, MIP1A, and TNF alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 3 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Information Description

Reactivity:

Rat

Buffers:

2x dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.

Analytes:

IL2, IL10, GSCF, IP10, MCP1, MIP1A, and TNFα

Lyophilized Standard Mix:

Lyophilized Standard Mix-Rat COVID-19 Multiplex Cytokine Immunoassay. One vial containing lyophilized recombinant IL2, IL7, IL10, GSCF, IP10, MCP1, MIP1A, and TNF alpha

Sample Types:

Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Standard dose recovery:

70-130%

Intra-assay CV:

< 10%

Inter-assay CV:

< 20%

Cross-reactivity of analytes in the Panel:

Negligible

Sample volume:

15 µL/test

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