The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

Rat BDNF ELISA Kit

(No reviews yet) Write a Review
SKU:
RTFI00006

Description

Rat BDNF ELISA Kit - Information

The ELISA Genie Rat BDNF (Brain Derived Neurotrophic Factor) ELISA Kit can assay for Rat BDNF (Brain Derived Neurotrophic Factor) in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Rat BDNF (Brain Derived Neurotrophic Factor) ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Rat BDNF (Brain Derived Neurotrophic Factor) is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Rat BDNF ELISA Kit - Data

Description

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.

Post-Translational Modification

Converted into mature BDNF by plasmin (PLG).

Uniprot ID P23363
Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of BDNF concentrations in serum plasma and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of BDNF and the recovery rates were calculated by comparing the measured value to the expected amount of BDNF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 95
EDTA plasma(n=5) 85-102 95
UFH plasma(n=5) 85-99 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 89-103% 89-105% 87-100%
EDTA plasma(n=5) 85-100% 87-100% 88-101% 89-100%
UFH plasma(n=5) 81-99% 82-98% 86-100% 81-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

View AllClose

Additional Information

Reactivity:
Rat
ELISA Type:
Sandwich
View AllClose