Mouse Interleukin 18 (IL18) SuperSet ELISA Kits
|Kit size:||20 plates|
|Alias:||IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma|
|Plate preparation:||1. Coat the plates with 100μL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.|
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
|Specificity||The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 18 (IL18). No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.|
|Sample type:||Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.|
|Database:||Entrez Gene: P97636 RGD:2889 Ensembl:ENSRNOG00000009848 Uniprot: P97636|
|Function – Uniprot:||Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.|
|Function – Entrez Gene:||a pro-inflammatory cytokine; acts as a modulator of immune functions [RGD, Feb 2006]|
|Subcellular Localization:||Extracellular region or secreted|
|Expression:||Ubiquitous expression in esophagus (RPKM 400.7), thyroid (RPKM 188.1) and 24 other tissues|
|Sequence similarities:||Belongs to the IL-1 family.|