Mouse Interleukin 1 Beta (IL1b) SuperSet ELISA Kits
|Kit size:||20 plates|
|Alias:||IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin|
|Plate preparation:||1. Coat the plates with 100μL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.|
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
|Specificity||The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b). No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.|
|Sample type:||Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.|
|Database:||Entrez Gene: Q63264 RGD:2891 Ensembl:ENSRNOG00000004649 Uniprot: Q63264|
|Function – Uniprot:||Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.|
|Function – Entrez Gene:||an inflammatory cytokine [RGD, Feb 2006]|
|Subcellular Localization:||Extracellular region or secreted; Cytosol; Lysosome; Other locations|
|Expression:||Ubiquitous expression in esophagus (RPKM 400.7), thyroid (RPKM 188.1) and 24 other tissues|
|Sequence similarities:||Belongs to the IL-1 family.|