Human MIP 3b / CCL19 ELISA Kit – Information
The ELISA Genie MIP 3b / CCL19 ELISA Kit can assay for MIP-3 beta / CCL19 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our MIP 3b / CCL19 ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound MIP-3 beta / CCL19 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Human MIP-3 beta / CCL19 ELISA Kit – Data
|Description||May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.|
|Alias||MIP-3 beta/ELC/CCL19/beta chemokine exodus-3/Beta-chemokine exodus-3/CC chemokine ligand 19/C-C motif chemokine 19/chemokine(C-C motif) ligand 19/CKb11/EBI1-ligand chemokine/ELCMIP-3-beta/Epstein-Barr virus-induced molecule 1 ligand chemokine/exodus-3/Macrophage inflammatory protein 3 beta/macrophage inflammatory protein 3-beta/MGC34433/MIP-3b/MIP3BCK beta-11/SCYA19EBI1 ligand chemokine/small inducible cytokine subfamily A(Cys-Cys) member 19/Small-inducible cytokine A19|
|Detection method||Sandwich ELISA Double Antibody|
|Application||This immunoassay kit allows for the in vitro quantitative determination of MIP-3 beta concentrations in serum plasma and other biological fluids.|
|Storage||4’C for 6 months|
|Recovery||Matrices listed below were spiked with certain level of MIP-3 beta and the recovery rates were calculated by comparing the measured value to the expected amount of MIP-3 beta in samples.
|Linearity||The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP-3 beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|Note||For Research Use Only|