Human Epidermal Growth Factor (EGF) SuperSet ELISA Kits
|Kit size:||20 plates|
|Alias:||URG; Beta-Urogastrone; Pro-epidermal growth factor|
|Plate preparation:||1. Coat the plates with 100μL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.|
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
|Specificity||The Abs in the kit have high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF). No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed.|
|Sample type:||serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids|
|Database:||Entrez Gene: P07522 RGD:2542 Ensembl:ENSRNOG00000053979 Uniprot: P07522|
|Function – Uniprot:||EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.|
|Function – Entrez Gene:||binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]|
|Subcellular Localization:||Other locations|
|Expression:||Ubiquitous expression in esophagus (RPKM 400.7), thyroid (RPKM 188.1) and 24 other tissues|
|Category:||calcium ion binding|