Human CD26 / DPP4 ELISA Kit – Information
The ELISA Genie CD26 / DPP4 ELISA Kit can assay for CD26 / DPP4 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our CD26 / DPP4 ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound CD26 / DPP4 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Human CD26 / DPP4 ELISA Kit – Data
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.1
The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing. N- and O-Glycosylated. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
CD26/DPP4/CD26/ADABP/ADCP-2/ADCP2DPP IV/Adenosine deaminase complexing protein 2TP103/CD26 antigen/CD26T-cell activation antigen CD26/dipeptidyl peptidase 4/Dipeptidyl peptidase IV/dipeptidylpeptidase 4/dipeptidyl-peptidase 4/dipeptidylpeptidase IV(CD26 adenosine deaminase complexing protein 2)/DPPIV
Sandwich ELISA Double Antibody
This immunoassay kit allows for the in vitro quantitative determination of CD26 concentrations in serum plasma and other biological fluids.
4’C for 6 months
Matrices listed below were spiked with certain level of CD26 and the recovery rates were calculated by comparing the measured value to the expected amount of CD26 in samples.
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD26 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
For Research Use Only