Canine Vitamin B12 (VB12) ELISA Kit
The ELISA Genie Canine Vitamin B12 (VB12) ELISA kit can assay for Canine Vitamin B12 (VB12) in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How do our ELISA Kits work?
The ELISA Genie Canine Vitamin B12 (VB12) ELISA Kits are designed for the competitive measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a competitive ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3’,5,5’-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Canine Vitamin B12 (VB12) is proportional to the signal generated by the reaction meaning the Canine Vitamin B12 (VB12) ELISA Kit assay gives you a quantitative measurement of the analyte in your samples.
Vitamin B12, VB12, cobalamin
CompetitiveCompetitive ELISA Technical Manual