Phospho-IRAK1 (Thr100) In-Cell ELISA

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Phospho-IRAK1 (Thr100) In-Cell ELISA

Product DescriptionThe Phospho-IRAK1 (Thr100) Colorimetric In-Cell ELISA Kit allows for qualitative detection of Phospho-IRAK1(Thr100) in plated and fixed cells.
Target SynonymsIL1RAK; IRAK; IRAK-1; Interleukin-1 receptor-associated kinase 1; Pelle-like protein kinase; kinase IRAK1; mPLK
Detection TargetPhospho-IRAK1(Thr209)
ReactivityH:T100, M:T100, R:T100
Pack Size2 x 96 assays
Detection MethodColorimetric 450 nm
FormatIn-Cell ELISA
Storage and Stability Guarantee4°C/6 Months
Sample TypePlated & fixed cells
Range5000 cells/well minimum
Time4.5 hours
SwissProt IDP51617
NCBI Gene ID3654

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Post-translational modifcation Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity.; Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation.

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