OCT1 Transcription Factor Activity Assay

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TFAB00145
€699

Description

ELISA Kit Technical ManualMSDS

OCT1 Transcription Factor activity assay

Product Description:The ELISA Genie Oct1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates ELISA Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:Organic cation transporter 1 Short name: hOCT1
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4°C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

OCT1 Transcription Factor activity assay Protein Information

UniProt Protein Function:SLC22A1: Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)- N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin- dependent kinase II and LCK tyrosine kinase. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function:protein binding; protein homodimerization activity; acetylcholine transmembrane transporter activity; norepinephrine transmembrane transporter activity; organic cation transmembrane transporter activity; secondary active organic cation transmembrane transporter activity; dopamine transmembrane transporter activity; quaternary ammonium group transmembrane transporter activity

Biological Process: dopamine transport; epinephrine transport; norepinephrine transport; organic cation transport; quaternary ammonium group transport; neurotransmitter transport; multidrug transport; establishment and/or maintenance of transmembrane electrochemical gradient; transmembrane transport; protein homooligomerization

NCBI Summary:Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. [provided by RefSeq, Jul 2008]
UniProt Code:O15245
NCBI GenInfo Identifier:313104181
NCBI Gene ID:6580
NCBI Accession:O15245.2
UniProt Related Accession:O15245
Molecular Weight:
NCBI Full Name:Solute carrier family 22 member 1
NCBI Synonym Full Names:solute carrier family 22 member 1
NCBI Official Symbol:SLC22A1
NCBI Official Synonym Symbols:OCT1; HOCT1; oct1_cds
NCBI Protein Information:solute carrier family 22 member 1
UniProt Protein Name:Solute carrier family 22 member 1
UniProt Synonym Protein Names:Organic cation transporter 1; hOCT1
UniProt Gene Name:SLC22A1
UniProt Entry Name:S22A1_HUMAN
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