Product Name: Genieplex NHP Th1/Th2/Th17 7-plex Kit
AnalytesIFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha
This GeniePlex Genieplex NHP Th1/Th2/Th17 7-plex Kit is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 7 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
|Analytes:||IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha|
|1x Ab-conjugated beads (premixed):||S4P3 - anti-NHP TNF-Alpha; S4P7 - anti-NHP IL-2; S4P9 - anti-NHP IFN-Gamma; S5P9 - anti-NHP IL-17A; S5P4 - anti-NHP IL-4; S5P6 - anti-NHP IL-6; S5P10 - anti-NHP IL-10|
|2x Biotin-detection Ab (premixed):||Biotin - anti-NHP TNF-Alpha; Biotin - anti-NHP IL-2; Biotin - anti -NHP IFN-Gamma; Biotin - anti-NHP IL-17A; Biotin - anti-NHP IL-4; Biotin - anti-NHP IL-6; Biotin - anti-NHP IL-10|
|Buffers:||2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.|
|Lyophilized Standard Mix:||Lyophilized Standard Mix-NHP Group 1 Panel A, 7-Plex. One vial containing lyophilized recombinant IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha.|
|Sample types:||Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate|
|Sensitivity (LODs):||IFN-Gamma, < 5 pg/mL; IL-2, < 5 pg/mL; IL-4 < 1 pg/mL, IL-6 <2 pg/mL; IL_10, < 2 pg/mL; IL-17A, < 2 pg/mL; TNF-Alpha, < 1 pg/mL|
|LLOQs:||IFN-Gamma, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4 < 5 pg/mL, IL-6 <5 pg/mL, IL-10, < 5 pg/mL; IL-17A, < 5 pg/mL; TNF-Alpha, < 2 pg/mL|
|ULOQs:||IFN-Gamma, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4 > 2,000 pg/mL; IL-6 > 5,000 pg/ml; IL-10, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; TNF-Alpha, > 5,000 pg/mL.|
|Standard dose recovery:||Standard dose recovery: 70-130%|
|Intra-assay CV:||Intra-assay CV: < 10%|
|Inter-assay CV:||Inter-assay CV: < 20%|
|Cross-reactivity of analytes in the Panel:||Cross-reactivity of analytes in the Panel: Negligible|
|Sample volume:||Sample volume: 15 µL/test|