NF-kappaB p65 (Phospho-Ser529) Transcription Factor Activity Assay

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TFAB00042
€699

Description

ELISA Kit Technical ManualMSDS

NF-kappaB p65 (Phospho-Ser529) Transcription Factor activity assay

Product Description:The ELISA Genie NF-kappaB p65 (Phospho-Ser529) transcription factor activity assay Kit components necessary for detection and qualitative analysis of NF-kappaB p65 (Phospho-Ser529) active transcription factors in eukaryotic nuclear or cell lysates. ELISA Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse, Rat
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4°C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

NF-kappaB p65 (Phospho-Ser529) Transcription Factor activity assay Protein Information

UniProt Protein Function:NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.
UniProt Protein Details:

Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function:actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway

NCBI Summary:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q04206
NCBI GenInfo Identifier:62906901
NCBI Gene ID:5970
NCBI Accession:Q04206.2
UniProt Secondary Accession:Q04206,Q6GTV1, Q6SLK1,
UniProt Related Accession:Q04206
Molecular Weight:59,910 Da
NCBI Full Name:Transcription factor p65
NCBI Synonym Full Names:RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol:RELA
NCBI Official Synonym Symbols:p65; NFKB3
NCBI Protein Information:transcription factor p65
UniProt Protein Name:Transcription factor p65
UniProt Synonym Protein Names:Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Protein Family:Proline-rich P65 protein
UniProt Gene Name:RELA
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