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Mouse TNF alpha ELISA Kit

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SKU:
MOFI00104

Description

Mouse TNF alpha ELISA Kit - Information

The ELISA Genie Mouse TNF-alpha ELISA Kit can assay for Mouse TNF-alpha in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Mouse TNF-alpha ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Mouse TNF-alpha is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Mouse TNF alpha ELISA Kit - Data

Alias TNF alpha
Detection method Sandwich ELISA Double Antibody
Application This immunoassay kit allows for the in vitro quantitative determination of TNF alpha(Tumor Necrosis Factor Alpha)/TNF-alpha/DIF/TNF-alpha/TNFA/TNFSF2 concentrations in serum plasma and other biological fluids.
Size 96T
Range 31.25-2000pg/ml
Sensitivity < 18.75pg/ml
Storage 4'C for 6 months
Recovery Matrices listed below were spiked with certain level of TNF alpha(Tumor Necrosis Factor Alpha)/TNF-alpha/DIF/TNF-alpha/TNFA/TNFSF2 and the recovery rates were calculated by comparing the measured value to the expected amount of TNF alpha(Tumor Necrosis Factor Alpha)/TNF-alpha/DIF/TNF-alpha/TNFA/TNFSF2 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5) 85-104 94
EDTA plasma(n=5) 86-103 97
UFH plasma(n=5) 91-102 96
Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF alpha (Tumor Necrosis Factor Alpha)/TNF-alpha/DIF/TNF-alpha/TNFA/TNFSF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:81:16
serum(n=5) 93-102% 90-105% 85-100% 87-102%
EDTA plasma(n=5) 86-101% 82-99% 82-93% 89-101%
UFH plasma(n=5) 80-96% 82-99% 81-89% 85-96%
CV(%) Intra-Assay: CV<8%Inter-Assay: CV<10%
Note For Research Use Only
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Additional Information

Reactivity:
Mouse
ELISA Type:
Sandwich
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