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Mouse PXR / NR1I2 ELISA Kit

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SKU:
MOFI00332

Description

Mouse PXR / NR1I2 ELISA Kit - Information

The ELISA Genie Mouse PXR / NR1I2 ELISA Kit can assay for Mouse PXR / NR1I2 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Mouse PXR / NR1I2 ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Mouse PXR / NR1I2 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Mouse PXR / NR1I2 ELISA Kit - Data

Description

Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. Activated by naturally occurring steroids such as pregnenolone and progesterone, the cholesterol metabolite 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol, synthetic glucocorticoids and antiglucocorticoids and 16-alpha-carbonitrile (PCN).

Post-Translational Modification

Uniprot ID O54915
Alias

Nr1i2

Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of Nr1i2/PXR/Pregnane X Receptor/NR1I2/Nuclear receptor subfamily 1 group I member 2/Steroid and xenobiotic receptor/SXR/Orphan nuclear receptor PAR1/Orphan nuclear receptor PXR concentrations in serum plasma and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of Nr1i2/PXR/Pregnane X Receptor/NR1I2/Nuclear receptor subfamily 1 group I member 2/Steroid and xenobiotic receptor/SXR/Orphan nuclear receptor PAR1/Orphan nuclear receptor PXR and the recovery rates were calculated by comparing the measured value to the expected amount of Nr1i2/PXR/Pregnane X Receptor/NR1I2/Nuclear receptor subfamily 1 group I member 2/Steroid and xenobiotic receptor/SXR/Orphan nuclear receptor PAR1/Orphan nuclear receptor PXR in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 96
EDTA plasma(n=5) 94-102 97
UFH plasma(n=5) 93-104 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nr1i2/PXR/Pregnane X Receptor/NR1I2/Nuclear receptor subfamily 1 group I member 2/Steroid and xenobiotic receptor/SXR/Orphan nuclear receptor PAR1/Orphan nuclear receptor PXR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 85-104% 86-104% 90-104%
EDTA plasma(n=5) 89-101% 84-101% 82-97% 86-98%
UFH plasma(n=5) 85-99% 89-100% 82-100% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
Mouse
ELISA Type:
Sandwich
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