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Mouse Interleukin 18 (IL18) SuperSet ELISA Kit

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SKU:
SSMO0071

Description

Mouse Interleukin 18 (IL18) SuperSet ELISA Kits

The Mouse Interleukin 18 (IL18) SuperSet ELISA Kit is a development ELISA kit for the detection of Interleukin 18 (IL18) in a range of sample types.

The ELISA Genie Interleukin 18 (IL18) SuperSet ELISA kits are a range of specially designed components for the development of your very own sandwich ELISA. Each kit contains an optimised antibody pair as well as recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA can also be used for the detection of analytes in more complex matrices such as serum and plasma. As with all ELISA development kits, it is essential to fully evaluate your sample type with the kit prior to experimentation.

Key Features

  • Optimised antibody pair for highly specific and sensitive analyte detection
  • High quality recombinant protein to generate consistent standard curves
  • Maximise laboratory budget with essential ELISA reagents for medium and high throughput analysis
  • Customise your own ELISA for your specific requirements
  • SuperSet ELISA Reagent Kit SSMO0071 enables full and easy assay optimisation
Kit size:20 plates
Range:15.6-1000pg/mL
Specificity:5.7pg/mL
Alias:IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
Plate preparation:1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
SpecificityThe Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 18 (IL18). No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.
Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Reactivity:Mouse
Database:Entrez Gene: P97636 RGD:2889 Ensembl:ENSRNOG00000009848 Uniprot: P97636
Gene Name:Il18
Gene ID:29197
Function - Uniprot:Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Function - Entrez Gene:a pro-inflammatory cytokine; acts as a modulator of immune functions
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Additional Information

Reactivity:
Mouse
ELISA Type:
SuperSet
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