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Mouse Interleukin 1 Beta (IL1b) SuperSet ELISA Kit

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SKU:
SSMO0366

Description

Mouse Interleukin 1 Beta (IL1b) SuperSet ELISA Kits

The Mouse Interleukin 1 Beta (IL1b) SuperSet ELISA Kit is a development ELISA kit for the detection of Interleukin 1 Beta (IL1b) in a range of sample types.

The ELISA Genie Interleukin 1 Beta (IL1b) SuperSet ELISA kits are a range of specially designed components for the development of your very own sandwich ELISA. Each kit contains an optimised antibody pair as well as recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA can also be used for the detection of analytes in more complex matrices such as serum and plasma. As with all ELISA development kits, it is essential to fully evaluate your sample type with the kit prior to experimentation.

Key Features

  • Optimised antibody pair for highly specific and sensitive analyte detection
  • High quality recombinant protein to generate consistent standard curves
  • Maximise laboratory budget with essential ELISA reagents for medium and high throughput analysis
  • Customise your own ELISA for your specific requirements
  • SuperSet ELISA Reagent Kit SSMO0366 enables full and easy assay optimisation
Kit size:20 plates
Range:15.6-1000pg/mL
Specificity:6.4pg/mL
Alias:IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
Plate preparation:1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
SpecificityThe Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b). No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.
Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Reactivity:Mouse
Database:Entrez Gene: Q63264 RGD:2891 Ensembl:ENSRNOG00000004649 Uniprot: Q63264
Gene Name:Il1b
Gene ID:24494
Function - Uniprot:Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Function - Entrez Gene:an inflammatory cytokine
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Additional Information

Reactivity:
Mouse
ELISA Type:
SuperSet
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