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Mouse IL-16 ELISA Kit

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SKU:
MOFI00928

Description

Mouse IL-16 ELISA Kit - Information

The ELISA Genie Mouse IL-16 ELISA Kit can assay for Mouse IL-16 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Mouse IL-16 ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Mouse IL-16 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Mouse IL-16 ELISA Kit - Data

Description

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.; Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.; Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

Post-Translational Modification

Synthesized as a chemo-attractant inactive precursor which is proteolytically cleaved by caspase-3 to yield IL-16.

Uniprot ID O54824
Alias

IL-16

Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of IL-16(Interleukin 16)/IL16/LCF/NIL16/PRIL16/prIL-16 concentrations in serum plasma and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-16(Interleukin 16)/IL16/LCF/NIL16/PRIL16/prIL-16 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-16(Interleukin 16)/IL16/LCF/NIL16/PRIL16/prIL-16 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 97-102 99
EDTA plasma(n=5) 94-103 99
UFH plasma(n=5) 92-103 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-16(Interleukin 16)/IL16/LCF/NIL16/PRIL16/prIL-16 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-99% 85-103% 92-105% 94-105%
EDTA plasma(n=5) 85-101% 85-99% 83-98% 82-100%
UFH plasma(n=5) 84-99% 84-99% 86-97% 82-92%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
Mouse
ELISA Type:
Sandwich
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