Human TNF alpha SuperSet ELISA Kit
Human TNF alpha SuperSet ELISA Kit
The Human TNF alpha SuperSet ELISA Kit is an ELISA kit for the detection of TNF-alpha in a range of sample types. SuperSet ELISA Kits are a range of specially designed ELISA Assays with the pharmaceutical and biotech research industries in mind. Focusing on the high quality monoclonal antibodies and purified standards, SuperSet ELISA Kits are key tools for immunology research. Each kit contains a highly optimised antibody pair as well as a purified recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA Kits can also be used for the detection of analytes in more complex matrices such as serum and plasma.
SuperSet ELISA Kit Key Features
- Highly optimised monoclonal antibody pair for highly specific and sensitive analyte detection.
- High quality purified recombinant protein to generate consistent standard curves.
- Accuracy and reliability are guaranteed as all reagents have been validated according ISO 9001:2000 quality systems.
- Recognises both Natural and Recombinant antigen Specificity.
- No cross reactivity with other cytokines tested.
- Standard Calibration to NIBSC.
- ELISA Kits developed with pharmaceutical and biotech research sectors in mind.
|Size:||1,5,10,15,20 x 96 Discovery|
|Alias:||APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha|
|Assay Range:||25 pg/ml - 800 pg/ml|
|Specificity:||Recognizes both natural and recombinant human TNF-a|
|Incubation:||From sample to end 2h45|
|SampleType:||Serum, Plasma, Cell culture supernatant|
|Sample Size:||100 µl|
|Cross Reactivity:||No cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.|
|Kit Contents:||SuperSet ELISA Kits include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.|
Materials required but not provided
- 96 well Microtiter plates: e.g. Nunc Maxisorp Cat # 468667 * Note: the use of ELISA plates which are not high affinity binding will result in lower performances.
- Reconstitution Buffer: 1xPBS, 0.09% Azide *
- Coating Buffer: 1xPBS, pH 7.2-7.4 *
- Wash Buffer: 1xPBS, 0.05% Tween20 *
- Blocking Buffer: 1xPBS, 5% BSA *
- Standard Dilution Buffer: 1xPBS, 1% BSA *
- Secondary Antibody Dilution Buffer: 1xPBS, 1% BSA * Note: Supplementation with 10% Animal Serum e.g. FCS for assaying serum, plasma or other body fluids samples may be necessary.
- HRP Dilution Buffer: 1xPBS, 1% BSA, 0.1% Tween20 *
- Stop Reagent: 1N Sulfuric Acid
* Available in an Accessory Pack for ELISA Set, ELISA Genie product RGDC00001, material for optimal performances and quantity provided for 5 plates.
- Microtitre plate reader with appropriate filters 450nm required with optional 630nm reference filter
- Microplate washer or wash bottle
- 10, 50, 100, 200 and 1,000µl adjustable single channel micropipettes with disposable tips
- 50-300µl multi-channel micropipette with disposable tips
- Multichannel micropipette reagent reservoirs
- Distilled water
- Vortex mixer
- Miscellaneous laboratory plastic and/or glass, if possible sterile