The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

GeniePlex Human Th1/Th2/Th17/Treg 10-Plex

(No reviews yet) Write a Review


Product Name: GeniePlex Human Th1/Th2/Th17/Treg 10-Plex Immunoassay Panel


G-CSF, GM-CSF, IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha and TNF-Beta

Species Reactivity:


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Human Th1/Th2/Th17/Treg 10-Plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes G-CSF, GM-CSF, IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha and TNF-Beta on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 10 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Analytes:G-CSF, GM-CSF, IFN-Gamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-Alpha and TNF-Beta
1x Ab-conjugated beads (premixed):S4P3 - anti-H IL-10; S4P4 - anti-H IL-6; S4P6 - anti-H TNFalpha; S4P7 - anti-H IL-2; S4P11 - anti-H TNFbeta; S5P2 - anti-H IFNgamma; S5P3 - anti-H GM-CSF; S5P4 - anti-H IL-4; S5P7 - anti-H IL-17A; S5P10 - anti-H G-CSF
2x Biotin-detection Ab (premixed):Biotin - anti-H IL-10; Biotin - anti-H IL-6; Biotin - anti-H TNFalpha; Biotin - anti-H IL-2; Biotin - anti-H TNFbeta; Biotin - anti-H IFNgamma; Biotin - anti-H GM-CSF; Biotin - anti-H IL-4; Biotin - anti-H IL-17A; Biotin - anti-H G-CSF
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix-Human TH1/Th2 10-Plex. One vial containing lyophilized recombinant G-CSF, GM-CSF, IFNgamma, IL-10, IL-17A, IL-2, IL-4, IL-6, TNFalpha and TNFbeta.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):G-CSF, < 5 pg/mL; GM-CSF, < 5 pg/mL; IFN-Gamma, < 3 pg/mL; IL-2, < 3 pg/mL; IL-4, < 1 pg/mL; IL-6, < 5 pg/mL; IL-10, < 2 pg/mL; IL-17A, < 3 pg/mL; TNF-Alpha, < 1 pg/mL; TNF-Beta, < 5 pg/mL.
LLOQs:G-CSF, < 10 pg/mL; GM-CSF, < 10 pg/mL; IFN-Gamma, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4, < 3 pg/mL; IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-17A, < 10 pg/mL; TNF-Alpha, < 20pg/mL; TNF-Beta, < 10 pg/mL.
ULOQs:G-CSF, > 5,000 pg/mL; GM-CSF, > 5,000 pg/mL; IFN-Gamma, > 2,000 pg/mL; IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 5,000 pg/mL; IL-17A, > 1,000 pg/mL; TNF-Alpha, > 1,000 pg/mL; TNF-Beta, > 5,000 pg/mL..
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test


ELISA Kit Technical Manual

View AllClose

Additional Information

View AllClose