The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

GeniePlex Human Th1/Th2/Th17 12-plex (96 Tests)

(No reviews yet) Write a Review
SKU:
HUAMPM028
$2,683

Description

Product Name: GeniePlex Human Th1/Th2/Th17 12-plex

Analytes

IFN-Gamma, IL-1-Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF-Beta

Species Reactivity:

Human

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Human Th1/Th2/Th17 12-plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-1-Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF-Beta on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 12 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Reactivity:Human
Analytes:IFN-Gamma, IL-1-Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF-Beta
1x Ab-conjugated beads (premixed):S4P2 - anti-human TNF-Beta; S4P3 - anti-human TNF-Alpha; S4P6 - anti-human IL-1-Beta; S4P7 - anti-human IL-2; S4P9 - anti-human IFN-Gamma; S4P10 - anti-human IL-8; S4P12 - anti-human IL-12p70; S5P4 - anti-human IL-4; S5P5 - anti-human IL-5; S5P6 - anti-human IL-6; S5P9 - anti-human IL-17A; S5P10 - anti-human IL-10
2x Biotin-detection Ab (premixed):Biotin - anti-human TNF-Beta; Biotin - anti-human TNF-Alpha; Biotin - anti-human IL-1-Beta; Biotin - anti-human IL-2; Biotin - anti -human IFN-Gamma; Biotin - anti-human IL-8; Biotin - anti-human IL-12p70; Biotin - anti-human IL-4; Biotin - anti-human IL-5; Biotin - anti-human IL-6; Biotin - anti-human IL-17A; Biotin - anti-human IL-10
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix- Human TH1/Th2/Th17 12-plex. Vial A and Vial B containing lyophilized recombinant human IFN-Gamma, IL-1 Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF Beta.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):IFN-Gamma, < 3 pg/mL; IL-1-Beta, < 5 pg/mL; IL-2, < 3 pg/mL; IL-4, < 1 pg/mL; IL-5, < 2 pg/mL; IL-6, < 5 pg/mL; IL-8, < 1 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-17A, < 3 pg/mL; TNF-Alpha, < 1 pg/mL; TNF-Beta, < 5 pg/mL.
LLOQs:IFN-Gamma, < 10 pg/mL; IL-1-Beta, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4, < 3 pg/mL; IL-5, < 5 pg/mL; IL-6, < 10 pg/mL; IL-8, < 1 pg/mL; IL-10, < 5 pg/mL;  IL-12p70, < 10 pg/mL; IL-17A, < 10 pg/mL; TNF-Alpha, < 2 pg/mL; TNF-Beta, < 10 pg/mL.
ULOQs:IFN-Gamma, > 2,000 pg/mL; IL-1-Beta, > 5,000 pg/mL;  IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mL; IL-5, > 2,000 pg/mL; IL-6, > 5,000 pg/mL; IL-8, > 1,000 pg/mL;  IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL;  IL-17A, > 1,000 pg/mL; TNF-Alpha, > 1,000 pg/mL; TNF-Beta,  > 5,000 pg/mL.
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test
View AllClose