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Human PIIINP / Procollagen III N-terminal Propeptide ELISA Kit

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SKU:
HUFI00727

Description

Human PIIINP / Procollagen III N-terminal Propeptide ELISA Kit - Information

The ELISA Genie PIIINP / Procollagen III N-terminal Propeptide ELISA Kit can assay for PIIINP / Procollagen III N-terminal Propeptide in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our PIIINP / Procollagen III N-terminal Propeptide ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound PIIINP / Procollagen III N-terminal Propeptide is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Human PIIINP / Procollagen III N-terminal Propeptide ELISA Kit - Data

AliasPIIINP/P3NP/N-Propeptide Of Type III Procollagen/Procollagen III Amino Terminal Propeptide
Detection methodSandwich ELISA Double Antibody
ApplicationThis immunoassay kit allows for the in vitro quantitative determination of PIIINP concentrations in serum plasma and other biological fluids.
Size96T
Range23.438-1500pg/ml
Sensitivity< 14.063pg/ml
Storage4'C for 6 months
RecoveryMatrices listed below were spiked with certain level of PIIINP and the recovery rates were calculated by comparing the measured value to the expected amount of PIIINP in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)86-10598
EDTA plasma(n=5)90-10395
UFH plasma(n=5)89-10498
LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIIINP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:81:16
serum(n=5)89-105%86-105%85-104%86-99%
EDTA plasma(n=5)83-95%82-95%82-96%90-100%
UFH plasma(n=5)80-93%81-99%80-99%85-99%
CV(%)Intra-Assay: CV<8%Inter-Assay: CV<10%
NoteFor Research Use Only
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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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