The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

Human Interleukin 6 (IL6) SuperSet ELISA Kit

(No reviews yet) Write a Review
SKU:
SSHU0394

Description

Human Interleukin 6 (IL6) SuperSet ELISA Kits

The Human Interleukin 6 (IL6) SuperSet ELISA Kit is a development ELISA kit for the detection of Interleukin 6 (IL6) in a range of sample types.The ELISA Genie Interleukin 6 (IL6) SuperSet ELISA kits are a range of specially designed components for the development of your very own sandwich ELISA. Each kit contains an optimised antibody pair as well as recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA can also be used for the detection of analytes in more complex matrices such as serum and plasma. As with all ELISA development kits, it is essential to fully evaluate your sample type with the kit prior to experimentation.

Key Features

  • Optimised antibody pair for highly specific and sensitive analyte detection
  • High quality recombinant protein to generate consistent standard curves
  • Maximise laboratory budget with essential ELISA reagents for medium and high throughput analysis
  • Customise your own ELISA for your specific requirements
  • SuperSet ELISA Reagent Kit SSHU0394 enables full and easy assay optimisation
Kit size:20 plates
Range:7.8-500pg/mL
Specificity:3.2pg/mL.
Alias:MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
Plate preparation:1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
SpecificityThe Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Reactivity:Human
Database:Entrez Gene: P20607 RGD:2901 Ensembl:ENSRNOG00000010278 Uniprot: P20607
Gene Name:Il6
Gene ID:24498
Function - Uniprot:Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Function - Entrez Gene:a cytokine involved in development and possibly in neurodegenerative processes
View AllClose

Additional Information

Reactivity:
Human
ELISA Type:
SuperSet
View AllClose