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Human Interferon Gamma (IFNg) SuperSet ELISA Kit

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SKU:
SSHU0148

Description

Human Interferon Gamma (IFNg) SuperSet ELISA Kits

The Human Interferon Gamma (IFNg) SuperSet ELISA Kit is a development ELISA kit for the detection of Interferon Gamma (IFNg) in a range of sample types.The ELISA Genie Interferon Gamma (IFNg) SuperSet ELISA kits are a range of specially designed components for the development of your very own sandwich ELISA. Each kit contains an optimised antibody pair as well as recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA can also be used for the detection of analytes in more complex matrices such as serum and plasma. As with all ELISA development kits, it is essential to fully evaluate your sample type with the kit prior to experimentation.

Key Features

  • Optimised antibody pair for highly specific and sensitive analyte detection
  • High quality recombinant protein to generate consistent standard curves
  • Maximise laboratory budget with essential ELISA reagents for medium and high throughput analysis
  • Customise your own ELISA for your specific requirements
  • SuperSet ELISA Reagent Kit SSHU0148 enables full and easy assay optimisation
Kit size:20 plates
Range:15.6-1000pg/mL
Specificity:6.3pg/mL
Alias:IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
Plate preparation:1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
SpecificityThe Abs in the kit have high sensitivity and excellent specificity for detection of Interferon Gamma (IFNg).No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Reactivity:Human
Database:Entrez Gene: P01581 RGD:2866 Ensembl:ENSRNOG00000007468 Uniprot: P01581
Gene Name:Ifng
Gene ID:25712
Function - Uniprot:Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Function - Entrez Gene:an immune molecule produced by T lymphocytes in response to mitogens or antigens
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Additional Information

Reactivity:
Human
ELISA Type:
SuperSet
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