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GeniePlex Human Inflammation 16-Plex (96 Tests)

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Product Name: GeniePlex Human Inflammation 16-Plex Immunoassay Panel


IFN-Gamma, IL-1-Alpha, IL-1-Beta, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1-Alpha, and TNF-Alpha

Species Reactivity:


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Human Inflammation 16-Plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-1-Alpha, IL-1-Beta, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1-Alpha, and TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 16 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Analytes:IFN-Gamma, IL-1-Alpha, IL-1-Beta, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1-Alpha, and TNF-Alpha
1x Ab-conjugated beads (premixed):S4P2 - anti-human MIP-1-Alpha; S4P3 - anti-human TNF-Alpha; S4P4 - anti-human IP-10; S4P6 - anti-human IL-1-Beta; S4P7 - anti-human IL-27; S4P9 - anti-human IFN-Gamma; S4P10 - anti-human IL-8; S4P11 - anti-human IL-31; S4P12 - anti-human IL-12p70; S5P1 - anti-human MCP-1; S5P2 - anti-human IL-1-Alpha; S5P6 - anti-human IL-6; S5P7 - anti-human IL-33; S5P9 - anti-human IL-17A; S5P10 - anti-human IL-10; S5P12 - anti-human IL-13
2x Biotin-detection Ab (premixed):Biotin - anti-human MIP-1-Alpha; Biotin - anti-human TNF-Alpha; Biotin - anti-human IP-10; Biotin - anti-human IL-1-Beta; Biotin - anti-human IL-27; Biotin - anti -human IFN-Gamma; Biotin - anti-human IL-8; Biotin - anti-human IL-31; Biotin - anti-human IL-12p70; Biotin - anti-human MCP-1; Biotin - anti-human IL-1-Alpha; Biotin - anti-human IL-6; Biotin - anti-human IL-33; Biotin - anti-human IL-17A; Biotin - anti-human IL-10; Biotin - anti-human IL-13
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix- Human Inflammation 16-Plex. Vials A, B and C containing lyophilized recombinant human IFN-Gamma, IL-1-Alpha, IL-1 Betaa, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, TNF-Alpha.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):IFN-Gamma, < 3 pg/mL; IL-1-Alpha, < 2 pg/mL; IL-1-Beta, < 5 pg/mL; IL-6, < 5 pg/mL; IL-8, < 1 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-13, < 5 pg/mL; IL-17A, < 3 pg/mL; IL-27, < 5 pg/mL; IL-31, < 5 pg/mL; IL-33, < 5 pg/mL; IP-10, < 3 pg/mL; MCP-1, < 5 pg/mL; MIP-1-Alpha, < 2 pg/mL; TNF-Alpha, < 1 pg/mL.
LLOQs:IFN-Gamma, < 10 pg/mL; IL-1-Alpha, < 5 pg/mL; IL-1-Beta, < 10 pg/mL; IL-6, < 10 pg/mL; IL-8, < 1 pg/mL; IL-10, < 5 pg/mL;  IL-12p70, < 10 pg/mL; IL-13, < 10 pg/mL; IL-17A, < 10 pg/mL; IL-27, < 10 pg/mL; IL-31, < 10 pg/mL; IL-33, < 10 pg/mL; IP-10, < 10 pg/mL; MCP-1, < 5 pg/mL; MIP-1-Alpha, < 5 pg/mL; TNF-Alpha, < 2 pg/mL.
ULOQs:IFN-Gamma, > 2,000 pg/mL; IL-1-Alpha, > 2,000 pg/mL; IL-1-Beta, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-8, > 1,000 pg/mL;  IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-13, > 5,000 pg/mL; IL-17A, > 1,000 pg/mL; IL-27, > 5,000 pg/mL; IL-31, > 5,000 pg/mL; IL-33, > 5,000 pg/mL; IP-10, > 2,000 pg/mL; MCP-1, > 5,000 pg/mL; MIP-1-Alpha, > 2,000 pg/mL; TNF-Alpha, > 1,000 pg/mL.
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test


ELISA Kit Technical Manual

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