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Human IFN alpha / Interferon Alpha ELISA Kit

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SKU:
HUFI02571

Description

Human IFN alpha / Interferon Alpha ELISA Kit - Information

The ELISA Genie IFN alpha / Interferon Alpha ELISA Kit can assay for IFN alpha / Interferon Alpha in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our IFN alpha / Interferon Alpha ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound IFN alpha / Interferon Alpha is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Human IFN alpha / Interferon Alpha ELISA Kit - Data

AliasIFN-alpha (Interferon Alpha)
Detection methodSandwich ELISA Double Antibody
ApplicationThis immunoassay kit allows for the in vitro quantitative determination of IFN-alpha concentrations in serum plasma and other biological fluids.
Size96T
Range15.625-1000pg/ml
Sensitivity< 9.375pg/ml
Storage4'C for 6 months
RecoveryMatrices listed below were spiked with certain level of IFN-alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-alpha in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)86-10594
EDTA plasma(n=5)89-10498
UFH plasma(n=5)86-9490
LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:81:16
serum(n=5)87-104%85-105%88-99%86-105%
EDTA plasma(n=5)87-100%84-99%82-100%88-101%
UFH plasma(n=5)83-94%82-100%80-96%84-95%
CV(%)Intra-Assay: CV<8%Inter-Assay: CV<10%
NoteFor Research Use Only
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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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