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Human gp340 ELISA Kit

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SKU:
HUFI01458

Description

Human gp340 ELISA Kit - Information

The ELISA Genie gp340 ELISA Kit can assay for gp340 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our gp340 ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound gp340 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Human gp340 ELISA Kit - Data

Description

May be considered as a candidate tumor suppressor gene for brain, lung, esophageal, gastric, and colorectal cancers. May play roles in mucosal defense system, cellular immune defense and epithelial differentiation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. May function as a binding protein in saliva for the regulation of taste sensation. Binds to HIV-1 envelope protein and has been shown to both inhibit and facilitate viral transmission. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly-sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell.

Post-Translational Modification

Highly N- and O-glycosylated. The O-glycans are heavily sulfated.

Uniprot ID Q9UGM3
Alias

DMBT1/Deleted in malignant brain tumors 1 protein/Surfactant pulmonary-associated D-binding protein/Salivary agglutinin/SAG/Glycoprotein 340/Gp-340/Hensin/GP340/muclin

Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of DMBT1 concentrations in serum plasma and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of DMBT1 and the recovery rates were calculated by comparing the measured value to the expected amount of DMBT1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-103 95
EDTA plasma(n=5) 85-103 91
UFH plasma(n=5) 87-105 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DMBT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 86-105% 87-105% 93-103%
EDTA plasma(n=5) 85-100% 83-101% 82-101% 85-101%
UFH plasma(n=5) 88-100% 83-100% 85-99% 81-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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