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Human Epidermal Growth Factor (EGF) SuperSet ELISA Kit

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SKU:
SSHU0049

Description

Human Epidermal Growth Factor (EGF) SuperSet ELISA Kits

The Human Epidermal Growth Factor (EGF) SuperSet ELISA Kit is a development ELISA kit for the detection of Epidermal Growth Factor (EGF) in a range of sample types.

The ELISA Genie Epidermal Growth Factor (EGF) SuperSet ELISA kits are a range of specially designed components for the development of your very own sandwich ELISA. Each kit contains an optimised antibody pair as well as recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA can also be used for the detection of analytes in more complex matrices such as serum and plasma. As with all ELISA development kits, it is essential to fully evaluate your sample type with the kit prior to experimentation.

Key Features

  • Optimised antibody pair for highly specific and sensitive analyte detection
  • High quality recombinant protein to generate consistent standard curves
  • Maximise laboratory budget with essential ELISA reagents for medium and high throughput analysis
  • Customise your own ELISA for your specific requirements
  • SuperSet ELISA Reagent Kit SSHU0049 enables full and easy assay optimisation
Kit size:20 plates
Range:3.9-250pg/mL
Specificity:1.5pg/mL
Alias:URG; Beta-Urogastrone; Pro-epidermal growth factor
Plate preparation:1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
SpecificityThe Abs in the kit have high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF). No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed.
Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Reactivity:Human
Database:Entrez Gene: P07522 RGD:2542 Ensembl:ENSRNOG00000053979 Uniprot: P07522
Gene Name:Egf
Gene ID:25313
Function - Uniprot:EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Function - Entrez Gene:binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways
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Additional Information

Reactivity:
Human
ELISA Type:
SuperSet
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