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Human EGF ELISA Kit

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SKU:
HUFI00009

Description

Human EGF ELISA Kit - Information

The ELISA Genie EGF ELISA Kit can assay for EGF in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our EGF ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound EGF is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Human EGF ELISA Kit - Data

Description

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Post-Translational Modification

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

Uniprot ID P01133
Alias

EGF(Epidermal growth factor)/URG/HOMG4/Beta-Urogastrone

Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of EGF concentrations in serum plasma and other biological fluids.

Size

96T

Range

3.906-250pg/ml

Sensitivity

< 2.344pg/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of EGF and the recovery rates were calculated by comparing the measured value to the expected amount of EGF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 94
EDTA plasma(n=5) 88-101 93
UFH plasma(n=5) 88-103 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 88-104% 86-103% 90-105%
EDTA plasma(n=5) 85-93% 92-97% 84-100% 83-95%
UFH plasma(n=5) 81-96% 85-97% 84-100% 83-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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