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Human CXCR3 / CD183 ELISA Kit

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SKU:
HUFI00746

Description

Human CXCR3 / CD183 ELISA Kit - Information

The ELISA Genie CXCR3 / CD183 ELISA Kit can assay for CXCR3 / CD183 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our CXCR3 / CD183 ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound CXCR3 / CD183 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Human CXCR3 / CD183 ELISA Kit - Data

Description

Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716). Binds to CCL21. Probably promotes cell chemotaxis response.; Isoform 2: Receptor for the C-X-C chemokine CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the proliferation, survival and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway (PubMed:12782716). Does not promote cell chemotaxis respons. Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis.; Isoform 3: Mediates the activity of CXCL11.

Post-Translational Modification

Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction. N-glycosylated.

Uniprot ID P49682
Alias

CXCR3/CD183/GPR9/CD183 antigen/chemokine(C-X-C motif) receptor 3/chemokine(C-X-C) receptor 3/CKR-L2IP-10 receptor/CMKAR3/C-X-C chemokine receptor type 3/CXC-R3/CXCR-3/G protein-coupled receptor 9/CD182/Mig-R/Interferon-inducible protein 10 receptor/IP10 receptor/IP10-R/Mig receptor/MigR

Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of CXCR3 concentrations in serum plasma and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of CXCR3 and the recovery rates were calculated by comparing the measured value to the expected amount of CXCR3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 94-104 97
EDTA plasma(n=5) 87-103 94
UFH plasma(n=5) 86-104 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CXCR3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 89-105% 87-105% 89-99%
EDTA plasma(n=5) 83-98% 82-101% 83-101% 82-90%
UFH plasma(n=5) 82-94% 84-97% 85-99% 83-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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