Product Name: GeniePlex Human Chemokine 6-Plex Immunoassay Panel
AnalytesEotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10
This GeniePlex GeniePlex Human Chemokine 6-Plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10 on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 6 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
|Analytes:||Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10|
|1x Ab-conjugated beads (premixed):||S4P1 - anti-human Eotaxin; S4P4 - anti-human IP-10; S4P10 - anti-human IL-8; S5P1 - anti-human MCP-1; S5P3 - anti-human MCP-3; S5P7 - anti-human RANTES|
|2x Biotin-detection Ab (premixed):||Biotin - anti-human Eotaxin; Biotin - anti-human IP-10; Biotin - anti-human IL-8; Biotin - anti-human MCP-1; Biotin - anti-human MCP-3; Biotin - anti-human RANTES|
|Buffers:||2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.|
|Lyophilized Standard Mix:||Lyophilized Standard Mix-Human Chemokine 6-Plex. One vial containing lyophilized recombinant Eotaxin, IL-8, IP-10, MCP-1, MCP-3, RANTES.|
|Sample types:||Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate|
|Sensitivity (LODs):||Eotaxin, < 5 pg/mL; IL-8, < 1 pg/mL; IP-10, < 3 pg/mL; MCP-1, < 2 pg/mL; MCP-3, < 2 pg/mL; RANTES, < 5 pg/mL.|
|LLOQs:||Eotaxin, < 10 pg/mL; IL-8, < 1 pg/mL; IP-10, < 10 pg/mL; MCP-1, < 5 pg/mL; MCP-3, < 5 pg/mL; RANTES, < 10 pg/mL.|
|ULOQs:||Eotaxin, > 2,000 pg/mL; IL-8, > 1,000 pg/mL; IP-10, > 2,000 pg/mL; MCP-1, > 5,000 pg/mL; MCP-3, > 5,000 pg/mL; RANTES, > 5,000 pg/mL..|
|Standard dose recovery:||Standard dose recovery: 70-130%|
|Intra-assay CV:||Intra-assay CV: < 10%|
|Inter-assay CV:||Inter-assay CV: < 20%|
|Cross-reactivity of analytes in the Panel:||Cross-reactivity of analytes in the Panel: Negligible|
|Sample volume:||Sample volume: 15 µL/test|