GeniePlex Human CD8+ T Cell Related Cytokines, 16-plex (96 Tests)

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SKU:
HUAMPM027
€3,049

Description

Product Name: GeniePlex Human CD8+ T Cell Related Cytokines, 16-plex Immunoassay Panel

Analytes

GM-CSF, Granzyme B, IFN-Gamma, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1-Alpha, MIP-1-Beta, RANTES, sCD137, sFasL, TNF-Alpha

Species Reactivity:

Human

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Human CD8+ T Cell Related Cytokines, 16-plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes GM-CSF, Granzyme B, IFN-Gamma, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1-Alpha, MIP-1-Beta, RANTES, sCD137, sFasL, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 16 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Reactivity:Human
Analytes:GM-CSF, Granzyme B, IFN-Gamma, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1-Alpha, MIP-1-Beta, RANTES, sCD137, sFasL, TNF-Alpha
1x Ab-conjugated beads (premixed):S4P2 - anti-human MIP-1-Alpha; S4P3 - anti-human TNF-Alpha; S4P6 - anti-human sCD137; S4P7 - anti-human IL-2; S4P8 - anti-human MIP-1-Beta; S4P9 - anti-human IFN-Gamma; S4P10 - anti-human Granzyme B; S4P11 - anti-human IL-15; S5P2 - anti-human sFasL; S5P3 - anti-human GM-CSF; S5P4 - anti-human IL-4; S5P5 - anti-human IL-5; S5P6 - anti-human IL-6; S5P7 - anti-human RANTES; S5P10 - anti-human IL-10; S5P12 - anti-human IL-13
2x Biotin-detection Ab (premixed):Biotin - anti-human MIP-1-Alpha; Biotin - anti-human TNF-Alpha; Biotin - anti-human sCD137; Biotin - anti-human IL-2; Biotin - anti-human MIP-1-Beta; Biotin - anti -human IFN-Gamma; Biotin - anti-human Granzyme B; Biotin - anti-human IL-15; Biotin - anti-human sFasL; Biotin - anti-human GM-CSF; Biotin - anti-human IL-4; Biotin - anti-human IL-5; Biotin - anti-human IL-6; Biotin - anti-human RANTES; Biotin - anti-human IL-10; Biotin - anti-human IL-13
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix- Human CD8+ T Cell Related Cytokines, 16-plex. One vial containing lyophilized recombinant GM-CSF, Granzyme B, IFN-Gamma, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1-Alpha, MIP-1 Beta, RANTES, sCD137, sFasL, TNF-Alpha.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):GM-CSF, < 5 pg/mL; Granzyme B, < 10 pg/mL; IFN-Gamma, < 3 pg/mL; IL-2, < 2 pg/mL; IL-4, < 1 pg/mL; IL-5, < 2 pg/mL; IL-6, < 5 pg/mL; IL-10, < 2 pg/mL; IL-13, < 5 pg/mL; IL-15, < 5 pg/mL; MIP-1-Alpha, < 2 pg/mL; MIP-1-Beta, < 5 pg/mL; RANTES, < 5 pg/mL; sCD137, < 5 pg/mL; sFasL, < 2 pg/mL; TNF-Alpha, < 1 pg/mL.
LLOQs:GM-CSF, < 10 pg/mL; Granzyme B, < 20 pg/mL; IFN-Gamma, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4, < 3 pg/mL; IL-5, < 5 pg/mL; IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-13, < 10 pg/mL; IL-15, < 10 pg/mL; MIP-1-Alpha, < 5 pg/mL; MIP-1-Beta, < 10 pg/mL; RANTES, < 10 pg/mL; sCD137, < 10 pg/mL; sFasL, < 5 pg/mL; TNF-Alpha, < 2 pg/mL.
ULOQs:GM-CSF, > 5,000 pg/mL; Granzyme B, > 5,000 pg/mL; IFN-Gamma, > 2,000 pg/mL; IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mL; IL-5, > 2,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 5,000 pg/mL; IL-13, > 5,000 pg/mL; IL-15, > 5,000 pg/mL; MIP-1-Alpha, > 2,000 pg/mL; MIP-1-Beta, > 5,000 pg/mL; RANTES, > 5,000 pg/mL; sCD137, > 5,000 pg/mL; sFasL, > 2,000 pg/mL; TNF-Alpha, > 1,000 pg/mL.
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test

Datasheets

ELISA Kit Technical Manual

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Additional Information

Reactivity:
Human
ELISA Type:
Multiplex
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