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Human Calbindin / CALB1 ELISA Kit

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SKU:
HUFI00983

Description

Human Calbindin / CALB1 ELISA Kit - Information

The ELISA Genie Calbindin / CALB1 ELISA Kit can assay for Calbindin / CALB1 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our Calbindin / CALB1 ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Calbindin / CALB1 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Human Calbindin / CALB1 ELISA Kit - Data

Description

Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Post-Translational Modification

Uniprot ID P05937
Alias

CALB1(Calbindin)/CAB27/Vitamin D-dependent calcium-binding protein avian-type/Calbindin D28/D-28K

Detection method

Sandwich ELISA Double Antibody

Application

This immunoassay kit allows for the in vitro quantitative determination of CALB1 concentrations in serum plasma and other biological fluids.

Size

96T

Range

6.25-400pg/ml

Sensitivity

< 3.75pg/ml

Storage

4'C for 6 months

Recovery

Matrices listed below were spiked with certain level of CALB1 and the recovery rates were calculated by comparing the measured value to the expected amount of CALB1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-104 95
EDTA plasma(n=5) 86-103 97
UFH plasma(n=5) 86-104 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CALB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-103% 91-104% 89-101% 90-103%
EDTA plasma(n=5) 83-101% 83-95% 87-100% 82-99%
UFH plasma(n=5) 80-91% 80-100% 80-88% 80-90%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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