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Human BDNF ELISA Kit

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SKU:
HUFI00039

Description

Human BDNF ELISA Kit - Information

The ELISA Genie BDNF (Brain Derived Neurotrophic Factor) ELISA Kit can assay for BDNF (Brain Derived Neurotrophic Factor) in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How our BDNF (Brain Derived Neurotrophic Factor) ELISA Kits Work?

The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.

At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3',5,5'-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound BDNF (Brain Derived Neurotrophic Factor) is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.

Human BDNF ELISA Kit - Data

Description During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.  
Post-Translational Modification The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG).
Uniprot ID P23560
Alias BDNF(Brain Derived Neurotrophic Factor)/ANON2/BULN2/Abrineurin/neurotrophin
Detection method Sandwich ELISA Double Antibody
Application This immunoassay kit allows for the in vitro quantitative determination of BDNF concentrations in serum plasma and other biological fluids.
Size 96T
Range 31.2-2000pg/ml
Sensitivity < 2pg/ml
Storage 4'C for 6 months
Recovery Matrices listed below were spiked with certain level of BDNF and the recovery rates were calculated by comparing the measured value to the expected amount of BDNF in samples.
MatrixRecovery range(%)Average(%)
serum(n=5) 90-101 95
EDTA plasma(n=5) 88-100 95
UFH plasma(n=5) 91-104 99
Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:81:16
serum(n=5) 92-105% 85-104% 86-105% 91-102%
EDTA plasma(n=5) 83-97% 82-90% 91-101% 82-94%
UFH plasma(n=5) 89-100% 80-97% 80-100% 82-92%
CV(%) Intra-Assay: CV<8%Inter-Assay: CV<10%
Note For Research Use Only
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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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