Human Amphiregulin (AREG) SuperSet ELISA Kits
|Kit size:||20 plates|
|Alias:||AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor|
|Plate preparation:||1. Coat the plates with 100uL per well of working solution of Capture Antibody. Incubate overnight at 4°C or incubate at 37°C for 2 hours.|
2. Aspirate and wash 1 time.
3. Block the plates with 200 uL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
|Specificity||The Abs in the kit have high sensitivity and excellent specificity for detection of Amphiregulin (AREG).No significant cross-reactivity or interference between Amphiregulin (AREG) and analogues was observed.|
|Sample type:||serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids|
|Database:||Entrez Gene: P15514 HGNC:HGNC:651 Ensembl:ENSG00000109321 Uniprot: P15514|
|Function - Uniprot:||Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.|
|Function - Entrez Gene:||The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions.|