Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) ELISA Kit

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SKU:
HUEB1680
€639

Description

ELISA Kit Technical ManualMSDS

ELISA Genie Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) ELISA Kit

The ELISA Genie Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) ELISA Kit can assay for Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.

How do our Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) ELISA Kits work?

The ELISA Genie Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase. During the reaction, in the sample or standard competes with a fixed amount of biotin-labeled for sites on a pre-coated Monoclonal antibody specific to Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene name

CNP

Protein Names/Synonym

2', 3'-cyclic-nucleotide 3'-phosphodiesterase, CNP, CNPase, CNP

Detection Range

0.312-20 ng/mL

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Additional Information

Reactivity:
Human
ELISA Type:
Sandwich
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