The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

Human 15-LO (Arachidonate 15-Lipoxygenase) CLIA Kit (HUES00173)

(No reviews yet) Write a Review
SKU:
HUES00173
$681

Description

Human 15-LO (Arachidonate 15-Lipoxygenase) CLIA Kit

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 15-LO . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 15-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human 15-LO. The concentration of Human 15-LO in the samples can be calculated by comparing the RLU of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityHuman
Detection methodChemiluminescence
Detection range15.63-1000 pg/mL
Sensitivity9.38 pg/mL
Sample volume100uL
Sample typeTissue homogenates,cell lysates and other biological fluids
RepeatabilityCV < 15%

Specificity

This kit recognizes Human 15-LO in samples. No significant cross-reactivity or interference between Human 15-LO and analogues was observed.

Typical Data

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(pg/mL)
RLU Average Corrected
100049087
58263
5367553650
50020753
24225
2248922464
25010868
9554
1021110186
1254669
5133
49014876
62.52567
2339
24532428
31.251346
1216
12811256
15.63703
713
708683
024
26
25--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human 15-LO were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human 15-LO were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)46.10155.71334.1150.64154.80344.54
Standard deviation4.7314.1720.285.9716.3235.90
C V (%)10.269.106.0711.7910.5410.42

Recovery

The recovery of Human 15-LO spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum (n=5)95-107101
EDTA plasma (n=5)86-9892
Cell culture media (n=5)99-110104

Linearity

Samples were spiked with high concentrations of Human 15-LO and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2Range (%)86-9885-10086-100
Average (%)939191
1:4Range (%)87-10283-9990-104
Average (%)949097
1:8Range (%)96-11096-11485-101
Average (%)10210492
1:16Range (%)103-119100-11597-112
Average (%)109107103

Kit Components & Storage

An unopened kit can be stored at 4'C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro CLIA Plate(Dismountable)8 wells ×12 strips-20'C, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 uL
Concentrated HRP Conjugate (100×)1 vial, 120 uL-20'C(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4'C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent A1 vial, 5 mL4'C (shading light)
Substrate Reagent B1 vial, 5 mL4'C (shading light)
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
View AllClose

Additional Information

Reactivity:
Human
ELISA Type:
CLIA
View AllClose