GeniePlex Human IL17F Immunoassay

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SKU:
HUAM00028
€239

Description

Product Name: GeniePlex Human IL-17F Immunoassay

Analyte

IL-17F

Species Reactivity:

Human

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex Human IL-17F Immunoassay is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of IL-17F on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify IL-17F in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

AnalytesIL-17F
ReactivityHuman
1x Ab-conjugated beads (premixed)S4P5
Sample types:Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs - pg/mL):< 2
LLOQ (pg/mL)< 5
ULOQ (pg/mL) > 2,000
Sample volume:Sample volume: 15 µL/test

Datasheets

ELISA Kit Technical Manual

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Additional Information

Reactivity:
Human
ELISA Type:
Multiplex
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