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ELISA Kits for Research

ELISA kits overview

At ELISA Genie we provide over 25,000 ELISA kits for research for a range of species including human, mouse, rat and many more. Our focus at ELISA Genie is to provide researchers the highest quality ELISA kits, the latest targets and new technologies that will allow researchers to push their immunology research further.


Top ELISA Kits

At ELISA Genie we are constantly developing ELISA kits for the measurement of key and the latest analytes. Searching publications and data to develop our latest kits, we focus on helping customers discover our future.

Human Insulin ELISA Kit
Human HIF-1 alpha / HIF1A ELISA Kit
Human CRP ELISA Kit
Human GDNF ELISA Kit

Human Leptin ELISA Kit
Human IGFBP-3 ELISA Kit
Human Catalase (CAT) ELISA Kit
Human Klotho ELISA Kit

ELISA Kit Example Data

Spike & recovery.

In order to asses the accuracy of an ELISA kit, spike and recovery and linear of dilution experiments provide important information in the validation and assessment of the accuracy of the ELISA kit assay.

The use of spike and recovery allows researchers to determine whether the detection of their analyte of interest is affected by the standard diluent and the biological sample matrix. In a spike and recovery experiment to assess and validate and ELISA kit a known amount of analyte (recombinant protein or hormone) is added into a test sample matrix and the response is compared to the identical spike into the standard diluent.

The example below of matrices listed below were spiked with certain level of IL8 and the recovery rates were calculated by comparing the measured value to the expected amount of IL8 in samples.

Matrix Recovery range(%) Average(%)

Serum(n=5)

88-101

95

EDTA plasma(n=5)

90-105

99

UFH plasma(n=5)

88-103

96

Linearity

In order to assess the precision for samples tested at different dilutions a linearity of dilution experiment is carried out. If the linearity of the assay is good over a range of dilutions (i.e. 1:2 to 1:16 for example), then the assay provides the ability to assay a sample with different levels of analyte. If a sample contains a high level of analyte, a sample may be diluted with standard dilution buffer and allow for comparison with an undiluted sample that contains low levels of the analyte of interest.

In the example below the linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16

Serum(n=5)

91-102%

87-101%

89-98%

92-104%

EDTA plasma(n=5)

89-100%

88-98%

83-92%

84-96%

UFH plasma(n=5)

83-96%

80-96%

80-100%

81-100%

Coefficient of variability (% CV)

ELISA kits are great tools to allow researchers to quantifiably measure the amount of analyte in a sample. However, in order to assess and show how well and ELISA assay kit is ran the results of the assay must a low coefficient of variability (%CV or CV). Whereby the %CV statistically shows the variance between replicate samples (Intra-assay) in an assay or between separately run assays (Inter-assay).

Analysis % CV

Intra-assay

<8%

Inter-assay

<10%


PharmaGenie ELISA Kits

PharmaGenie ELISA Kits from ELISA Genie are high quality ELISA Kits designed to meet the needs of pharma and biotech research. Focussing on high quality monoclonal antibody pairs & reagents that have been been validated according ISO 9001:2000 quality systems, PharmaGenie ELISA Kits are excellent assays to help discover our future.

High Quality Monoclonal Pairs

Control of our own mAb development coupled with robust manufacturing procedures ensures excellent performance and batch to batch consistency.

High Sensitivity Assays

High Sensitivity (HS) kits for the detection of low expressing proteins with sensitivities below 1pg/ml.

HIGH PERFORMANCE

  • Highly sensitive assay
  • Fast procedure
  • Accuracy and reliability are guaranteed as all our reagents have been validated according ISO 9001:2000 quality systems
  • Recognises both Natural and Recombinant antigen Specificity
  • No cross reactivity with other human cytokines tested
  • Standard Calibration to NIBSC
Human IL-6 PharmaGenie ELISA Kit
Human IL-2 PharmaGenie ELISA Kit

Cytokine ELISA kits

Human Cytokine ELISA Kits

At ELISA Genie we provide a huge range of cytokine ELISA kits for human, mouse, rat and many more species. Our range of cytokine ELISA kits cover key Interleukins, Interferons, TNF family members and more. Further ELISA Genie provide a small subset range of high sensitivity cytokine ELISA kits.

High Sensitivity Cytokine ELISA kits

Our PharmaGenie high sensitivity cytokine ELISA kits use high quality monoclonal antibodies to detect Interleukin 2 to 12 and IFN-gamma at less than <1pg/mL. The high sensitivity ELISA kits are a fast protocol that allows researchers to get the results they need with a protocol of 3 hours.

Features and Benefits

  • Less than 1pg/mL sensitivity
  • Key Interleukins
  • Fast protocol of ~3 hours
  • Ship at room temperature and eco-friendly
Analyte Range Sensitivity

1.87 pg/ml - 60 pg/ml

0.97 pg/ml

0.31 pg/ml - 10 pg/ml

0.31 pg/ml

1.56 pg/ml - 50 pg/ml

0.8 pg/ml

1.56 pg/ml - 50 pg/ml

1.30 pg/ml

0.78 pg/ml - 25 pg/ml

0.75 pg/ml

0.78 pg/ml - 25 pg/ml

0.69 pg/ml


SuperSet Development ELISA Kits (antibody pairs)

SuperSet Development ELISA kits allow researchers to prepare and plate their own ELISA plates using antibody pairs. SuperSet Development ELISA kits from ELISA Genie are high quality ELISA kits with sensitivity of <5pg/ml. These kits are made with high quality monoclonal antibodies to allow for increased sensitivity and specificity.

Features and benefits

 
  • Highly optimised monoclonal antibody pair.
  • High quality purified recombinant protein to generate consistent standard curves.
  • Reagents have been validated according ISO 9001:2000 quality systems.
  • Natural and Recombinant antigen Specificity.
  • No cross reactivity with other cytokines tested.
  • Standard Calibration to NIBSC.
  • ELISA Kits developed with pharmaceutical and biotech research sectors in mind.

SuperSet Development ELISA Kit Protocol Schematic

Figure: SuperSet developemt ELISA kits protocol. Coats wells with antibody & incubate overnight at 4'C. Add blocking buffer. Incubate for 120 mins at room temperature. Wash wells. Add standard and samples, add Biotin detection working solution. Incubate for 60 mins at RT. Wash wells. Add Step.-HRP. Wash wells. Add TMB substrate & incubate for 5-15 mins. Add stop solution and read at 450nm.


PharmaGenie Therapeutic Antibodies & Biosimilar ELISA Kits

The ELISA Genie Therapeutic Antibody, Biosimilar & Anti-Drug Antibody (ADA) ELISA kits are a premium range of kits designed to detect free therapeutic antibodies, biosimilars and Anti-Drug Antibodies in serum & plasma samples.

These kits use the highest quality components and are manufactured under ISO 13485 quality systems according to CE IVD standards.

Premium Quality Validated Assays

 
  • Manufactured in Europe under ISO 13485 quality systems & according to CE IVD standards.
  • Kits for Research-Use-Only.
  • Inter and intra assay CV meet requirements for FDA & EMEA
    Validation, verification and lot release performed according to best-in-class scientific guidelines and literature

Quantitative & Qualitative Screening Kits

 
  • Quantitative ELISA kits for the measurement of the World’s leading
    therapeutic antibodies & biosimilars
  • Qualitative kits enabling the screening of deleterious ADAs
  • Dual kits allowing for the screening of ADAs and free therapeutic antibodies

Optimized Format & Protocol

 
  • Use as little as 10ul sample per well – excellent for clinical, mouse, rat & primate samples
  • Get your results in as little as 55 minutes for some kits
  • All kits shipped at room temperature
Trastuzumab (Herceptin®, Herclon®) (Free drug) ELISA Kit
Bevacizumab (Avastin®) (Free drug) ELISA Kit
Infliximab (Remsima®) (Free drug) ELISA Kit

Mutliplex ELISA Kits

Multiplex ELISA technology allows researchers to measure a number of analytes in a single sample simultaneously. The ELISA Genie, GeniePlex multiplex assay technology utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With multiple sizes of beads and multiple levels of fluorescence intensity in each bead size, the GeniePlex technology can measure up to 24 analytes simultaneously using as little as 15μL.

The bead populations in the reaction are determined by a flow cytometer equipped with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700 nm.

Figure: Standard curvers of Human 18-plex Multiplex ELISA kit assay

Features & Benefits

  • Analyze: Multiplex 2-24 analytes in a single sample!
  • Fast: 2 hour protocol!
  • Sensitive: Measure as low as <10 pg/ml of each analyte
  • Dynamic: Lower limit < 20 pg/mL | Upper limit > 5,000 pg/mL
  • High Precision Intra-assay CV: < 10% | Inter-assay CV: < 20%
  • Low Volumes: Use as little as 15ul of sample
  • Validated: All assays fully tested for cross-reactivity in our lab

Multiplex ELISA kit protocol video

Multiplex ELISA Kit panels