The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

ELISA Dictionary


ELISA Keywords

This short article aims to identify and explain the common ELISA keywords you are likely to encounter when reading an ELISA protocol.

  • Solid Phase– Normally a microtiter plate well. Specially prepared ELISA plates are available commercially and be found on
  • Adsorption– This is the process of adding an antigen or antibody so that it attaches passively to the solid phase on incubation.
  • Washing– Is the flooding of the wells with buffered solution. This remove unbound reagents.
  • Antigen– A substance usually a protein which induces an immune response in the form of antibodies. The antibody produced is specific to the antigen it encounters.
  • Antibody– Produced in response in to antigenic stimuli. Mainly protein.
  • Enzyme– A substance which acts as a catalyst to promote a specific reaction. Enzymes act on specific substrates.
  • Enzyme conjugate– A enzyme which is attached to a protein usually an antibody such as a mouse anti-rabbit linked to HRP (horseradish peroxidase).
  • Substrate– A compound which reacts specifically with an enzyme. This reaction in ELISA produces a signal detectable directly by colour change.
  • Chromophore– A chemical that alters colour as a result of the enzyme interaction with substrate.
  • Stop solution– Stops the action of an enzyme on a substrate, stopping any further colour change.
  • Reading– Measurement of color produced in the ELISA. This is quantified using a spectrophotometer reading at specific wavelengths for the specific colours obtained with particular enzyme/chromophore systems.