Cytokine Storm Multiplex Panel 7-plex

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SKU:
HUAMCOV03
€1,799

Description

ELISA Kit Technical Manual

Cytokine Release Syndrome Multiplex Panel 7-plex

Analytes

IL-6, IL-10, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and CCL2/SYCA2/MCP-1

Species Reactivity:

Human

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

Cytokine Release Syndrome Multiplex Panel 7-plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IL-6, IL-10, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and CCL2/SYCA2/MCP-1 on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 3 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Reactivity: Human
Buffers: 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Analytes: IL-6, IL-10, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and CCL2/SYCA2/MCP-1
Lyophilized Standard Mix: Lyophilized Standard Mix-Human COVID-19 Multiplex Cytokine Immunoassay. One vial containing lyophilized recombinant IL-6, IL-10, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and CCL2/SYCA2/MCP-1
Sample types: Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Standard dose recovery: Standard dose recovery: 70-130%
Intra-assay CV: Intra-assay CV: < 10%
Inter-assay CV: Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume: Sample volume: 15 µL/test
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Additional Information

Reactivity:
Human
ELISA Type:
Multiplex
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